Keywords : UTI
Effect of Titanium Nanoparticles Biosynthesized by Lactobacillus crispatus against Multi Drug Resistance Bacteria isolated from patient with Recurrent UTI
Kirkuk Journal of Medical Sciences,
2018, Volume 6, Issue 1, Pages 49-65
Background: The synthesis of metal nanoparticles using microorganisms has received great interest due to their optical, chemical, photoelectrical and electronic properties. The microorganisms are used as possible ―nanofactories‖ for development of clean, nontoxic and environmentally friendly methods for producing nanoparticles, the Nanoparticle are synthesized using various biosources such as bacteria, fungi, yeast, plant extract Objectives: The present study focuses on the biosynthesis of titanium nanoparticles using Iraqi Lactobacillus crispatus isolate and their inhibitory effect against multi drug resistance bacteria isolated from patient with Recurrent UTI and against their ESBL and Metallo beta lactamase. Materiales and Methods: Lactobacillus crispatuswas isolated from vagina of Iraqi healthy women then identified throughoutcultural, microscopical and biochemical test. Fifty samples of urine were collected from women suffering from recurrent UTI who had certain clinical symptoms; these samples were collected from Rizgary Teaching Hospital and Baghdad Teaching Hospital from August to September, 2013 with ages ranging from 18-60 years. The urine specimens were inoculated on both blood and MacConkeys agar plates. The isolates were identified throughout cultural, microscopical, biochemical test and Vitek 2 system. Isolates were tested against 14 antibiotics by using Kirby-Bauer disc diffusion and detection of Extend spectrum β- lactamases by double disc diffusion synergy method and detection of Metallo-β lactamase by disc potentiating test and Samples of synthesized nanoparticles were characterized after 72 hours of incubation. The formation of metal oxide TiO2 nanoparticles was confirmed by X-ray diffraction (XRD) technique, Atomic Force Microscopy (AFM) and Scanning electron microscopic (SEM) and antibacterial activity of TiO2 nanoparticles synthesized by L. crispatus were determined on the basis of minimum inhibitory concentration (MIC) values Effect of TiO2 nanoparticles on metallo-β lactamase and evaluation of combined effect between Antibiotics and synthesized TiO2 nanoparticles. Results: Fifty samples of urine were collected from patient suffering from recurrent UTI who had certain clinical symptoms. Forty four isolates (88%) were isolated and identified from fifty samples of urine, 13 isolates (29.5%) belonged to Escherichia coli which was the most predominant and 6 isolates (13.6%) belonged to Klebsiella pneumonia, 5 isolates (11.3%) belonged to Psudomonasaeruginosa , 2 isolates (4.5%) belonged to Acinetobacterbaumannii, one isolate belonged to M.morganii, 11 isolates (25%) belonged to S.aureusand 6 isolates (13.6%) belonged to S.saprophyticusMoreover , these isolates were tested for susceptibility to (13) antibiotics and for their ability to produce extended β –lactmase and metalo β- lactmaseenzymes. All isolates were resist to Ampicillin, Amoxicillin/ clavulanicacid and Aztreonam. Inhibitory effect of synthesized TiO2 nanoparticles was studied against growth and ESBL and Metallo beta lactamase of recurrent UTI causative bacteria. Results showed the MIC of TiO2 nanoparticles was (32) mg\ml against E. coli, M.morganii, A.baumanii and S.aureus, and (64) mg\ml against K.pneumoniae, P.aeruginosa and S.saprophyticus isolates.TiO2 nanoparticles showed inhibitory effect on metalloβ lactmase produced by E.coli, M.morganiiand A.baumanii., thereduction in inhibition zone diameter of MBL positive isolates of A.baumanii(1) and E.colifrom (2o to 25) mm after treatment with TiO2 nanoparticles compared to control without treatment with TiO2 nanoparticles (25-35,20- 30) mm respectively, while inhibition zone diameters of M.morganiiand A.baumanii (2) were(15,20),(10,20) mm respectively after treatment compared to control with inhibition zone diameters (23,30), (30,38) mm. The combined effect between Antibiotics and synthesized TiO2 nanoparticles was also investigated against multi resistant recurrent UTI causative bacteria. The antibacterial activities of some antibiotics such as Ampicillin and Gentamycin have been increased in the presence of TiO2 nanoparticle (subMIC) against all isolates. Conclusions: Titanium nanoparticles has more efficient antibacterial agent and can be useful in the treatment of infectious diseases caused bybacteria, the combination of antibiotics and metal nanoparticles could increase the antibiotics‘ efficacy against resistant pathogens. In addition, nanoparticle–antibiotic conjugates lower the amount of both agents in the dosage, which reduces harmfulness and increases antimicrobial properties.
Kirkuk Journal of Medical Sciences,
2013, Volume 1, Issue 2, Pages 14-22
Background: Urinary tract infection (UTI) is the most common of all bacterial infections in diabetes mellitus; it affects persons throughout their life spans. The term UTI encompasses a variety of clinical entities ranging from asymptomatic bacteriuria to cystitis, prostatitis and pyelonephritis. Patients and methods: In the present study, 200 diabetic women,100 of them were at active age group(AAG) their ages range from (25-35)years with mean age of 31 years. Another 100 women were post-menopausal (PM) their ages range from (55-75) years with mean age of 64 years. Two hundred non diabetic women were taken as control group and were divided into 100 at AAG and 100 PM. The patients and control groups were attendant of Kirkuk general hospital. Results: UTI was found to be present in 28 out of 100 cases of AAG diabetic women (p value =0.02) while it presents in 25 out of 100 PM diabetic women (p value =0.04). Also this study shows that 13 out of 25 PM diabetic women received insulin for treatment while 7 out of 25 PM diabetic women received oral antidiabetic drugs, and only 5 out of 25 PM diabetic women didn't receive any treatment (p value=0.03). The present findings shows that 14 out of 28 AAG diabetic women received insulin for treatment, while 8 out of 28 of them received oral antidiabetic drugs and only 6 out of 28 AAG diabetic women didn't receive any treatment (p value =0.043) Conclusion: The prevalence of UTI is higher among diabetic patient compared to nondiabetic patient regardless of age .Diabetes treated with insulin is related to substantial increases in the risk of UTI among both age groups included in the present study